abstract：:In normal human fibroblasts, the synthesis of a cell surface receptor for plasma low density lipoprotein (LDL) is regulated by a sensitive system of feedback suppression. The number of functional LDL receptors declines by more than 20 fold when cellular stores of esterified cholesterol are increased by incubation of c...

journal_title：Cell

authors： Goldstein JL,Sobhani MK,Faust JR,Brown MS

更新日期：1976-10-01 00:00:00

abstract：:We have produced a series of somatic cell hybrids by fusing pluripotent PCC4aza1 embryonal carcinoma ("teretocarcinoma") cells with thymocytes from young adult mice. When these hybrids form tumors in nu/nu or syngeneic mice, all the tumors contain a range of differentiated tissues, as well as embryonal carcinoma-like ...

journal_title：Cell

authors： Miller RA,Ruddle FH

更新日期：1976-09-01 00:00:00

abstract：:Embryonal carcinoma cells derived from murine teratocarcinomas are able to differentiate into the same variety of tissue types as early embryonic cells. Because embryonal carcinoma cells resemble those of the embryo at a stage before X chromosome inactivation has occurred in females embryonal carcinoma cells containin...

journal_title：Cell

authors： McBurney MW,Adamson ED

更新日期：1976-09-01 00:00:00

abstract：:Histone DNA of Psammechinus miliaris was obtained in an enriched form by buoyant density gradient centrifugation and was cleaved into 6 kb repeat units (Birnstiel et al., 1975a) by the action of the specific endonucleases EcoRI and HindIII. Since it was suspected that the 6 kb unit harbored all five histone-coding seq...

journal_title：Cell

authors： Schaffner W,Gross K,Telford J,Birnstiel M

更新日期：1976-08-01 00:00:00

abstract：:Clonal derivatives of C3HMT murine mammary cell lines in culture demonstrate conversion of mammary tumor virus (MMTV) expression at a rate of appriximately 6 per 100 clones. This alteration is largely unidirectional from a relatively high level (MMTV(H)) to a 10 fold lower level (MMTV(L)). This high rate of MMTV(L) va...

journal_title：Cell

authors： Parks WP,Hubbell ES,Goldberg RJ,O'Neill FJ,Scolnick EM

更新日期：1976-05-01 00:00:00

abstract：:Nuclear DNA from eucaryotes contains a significant fraction which forms duplexes very rapidly and also independently of the DNA concentration. This fraction can be isolated by adsorption to hydroxylapatite and has been called foldback DNA (Britten and Smith, 1970). Here we extend previous studies to show that the fold...

journal_title：Cell

authors： Perlman S,Phillips C,Bishop JO

更新日期：1976-05-01 00:00:00

abstract：:The small molecular weight RNAs of the HeLa cell have been located in specific subcellular fractions. SnA is located in the nucleolus and is partially bonded to nucleolar 28S RNA. SnD, the most abundant of the small nuclear RNAs, is partially released from the nucleus when the nuclear preparation is briefly warmed. Sn...

journal_title：Cell

authors： Zieve G,Penman S

更新日期：1976-05-01 00:00:00

abstract：:Recombinant DNAs containing the E. coli plasmid pSC101 and mouse cell (La9) mitochondrial DNA (mtDNA) were formed in vitro via ligation of DNA fragments from limit EcoRI endonuclease digests and were used to transform E. coli K12. Four structurally different recombinant plasmid DNAs from transformed clones were charac...

journal_title：Cell

authors： Brown WM,Watson RM,Vinograd J,Tait KM,Boyer HW,Goodman HM

更新日期：1976-04-01 00:00:00

abstract：:We have analyzed the viral DNA sequences persisting in HE C19, a clonal line of hamster embryo cells transformed in vitro by human adenovirus 12 (Ad 12), a member of highly oncogenic group A. Saturation-hybridization of in vitro labeled Ad 12 DNA (2-4 x 10(8) cpm/mug) with HE C19 DNA showed that 93-100% of viral DNA s...

journal_title：Cell

authors： Green MR,Chinnadurai G,Mackey JK,Green M

更新日期：1976-03-01 00:00:00

abstract：:Physiological concentrations of dihydrotestosterone (DHT) can specifically induce the release of fibrinolysin from androgen-dependent mammary carcinoma (Shionogi SC-115) cells in vitro. No fibrinolytic activity was observed in cells cultured either in the absence of DHT or presence of pharmacological concentrations of...

journal_title：Cell

authors： Mak TW,Rutledge G,Sutherland DJ

更新日期：1976-02-01 00:00:00

abstract：:The majority of the mRNA molecules in HeLa cells contain 1-2 residue(s) of m6Ap and one blocked, methylated 5' terminal "cap" structure. The hnRNA, which is longer than mRNA, contains both m6Ap and caps but 4-6 times as many m6Ap residues per chain. In addition, nuclear molecules contain T2 RNA ase-resistant, methyl-l...

journal_title：Cell

authors： Salditt-Georgieff M,Jelinek W,Darnell JE,Furuichi Y,Morgan M,Shatkin A

更新日期：1976-02-01 00:00:00

abstract：:Organ cultures of liver from untreated male Xenopus respond to 17 beta-estradiol in the culture medium by synthesizing and secreting the yolk protein precursor vitellogenin. Vitellogenin synthesis, as a primary response, is first detectable on the fourth day of culture, and comprises up to 12% of the protein synthesiz...

journal_title：Cell

authors： Green CD,Tata JR

更新日期：1976-01-01 00:00:00

abstract：:The sulfated glycosaminoglycan, heparin, was found to release 125I-labeled low density lipoprotein (125I-LDL) from its receptor site on the surface of normal human fibroblasts. Measurement of the amount of 125I-LDL released by heparin permitted the resolution of the total cellular uptake of 125I-LDL at 37 degrees C in...

journal_title：Cell

authors： Goldstein JL,Basu SK,Brunschede GY,Brown MS

更新日期：1976-01-01 00:00:00

abstract：:Initiator tRNAs from the cytoplasm of salmon testes and salmon liver have been purified. The nucleotide sequence of these initiator tRNAs has been determined and found identical to that of initiator tRNA from mammalian cytoplasm. The only difference is the extent of modification of the nuceloside located between the d...

journal_title：Cell

authors： Gillum AM,Urquhart N,Smith M,RajBhandary UL

更新日期：1975-11-01 00:00:00

abstract：:From a transplantable mouse teratoma it has been possible to derive an established keratinizing cell line (XB) which grows well in cultures containing lethally irradiated 3T3 fibroblasts at the correct density. Single cells of the keratinizing line grow into colonies each consisting of a stratified squamous epithelium...

journal_title：Cell

authors： Rheinwald JG,Green H

更新日期：1975-11-01 00:00:00

abstract：:Purified reovirus synthesizes in vitro a mixture of mRNA molecules that contain 5' terminal structures of the type ppG..., GpppG..., and m7GpppGm, the relative amount of each depending upon the presence of S-adenosylmethionine (SAM) or S-adenosylhomocysteine (SAH) in the transcription incubation mixture. Reovirus mRNA...

journal_title：Cell

authors： Both GW,Furuichi Y,Muthukrishnan S,Shatkin AJ

更新日期：1975-10-01 00:00:00

abstract：:The relationship between activation of resting chick embryo fibroblasts by proteases and proteolytic alteration of the cell surface has been investigated. Five different proteases were examined: trypsin, collagenase, plasmin, alpha-chymotrypsin, and thrombin. All of these proteases, when added to the culture medium at...

journal_title：Cell

authors： Blumberg PM,Robbins PW

更新日期：1975-10-01 00:00:00

abstract：:Hybrids between mouse hepatoma cells (which secrete several serum proteins) and mouse or rat fibroblasts (which do not secrete these proteins) produce transferrin and the third component of complement (C3) like the parental hepatoma cells, while they do not secrete either albumin or alpha-fetoprotein (AFP). This lack ...

journal_title：Cell

authors： Szpirer J,Szpirer C

更新日期：1975-09-01 00:00:00

abstract：:The origin of a low molecular weight virus-associated RNA (VA-RNA) was mapped by hybridization of VA-RNA to specific fragments of adenovirus type 2 DNA, obtained after cleavage with three different restriction endonucleases. VA-RNA was found to hybridize exculsively to the l-strand [strand with low buoyant density in ...

journal_title：Cell

authors： Pettersson U,Philipson L

更新日期：1975-09-01 00:00:00

abstract：:Mitochondrial DNA replication was examined in mutants for seven different Saccharomyces cerevisiae genes which are essential for nuclear DNA replication. In cdc8 and cdc21, mutants defective in continued replication during the S phase of the cell cycle, mitochondrial DNA replication ceases at the nonpermissive tempera...

journal_title：Cell

authors： Newlon CS,Fangman WL

更新日期：1975-08-01 00:00:00

abstract：:Short DNA chains, isolated from in vitro pulse-labeled replicating polyoma DNA, exhibit some degree of self-complementarity (28% resistance to S1 nuclease after self-annealing to plateau levels). This level of self-annealing is not increased if short DNA chains present as free single-stranded DNA after extraction are ...

journal_title：Cell

authors： Francke B,Vogt M

更新日期：1975-06-01 00:00:00

abstract：:Long pyrimidine tracts, purified from Drosophila melanogaster DNA after treatment with formic acid-diphenylamine, were used as template for E. coli RNA polymerase to produce a polynucleotide containing only purines. This polypurine RNA hybridized specifically to D. melanogaster DNA with high efficiency at low Cot valu...

journal_title：Cell

authors： Sederoff R,Lowenstein L

更新日期：1975-06-01 00:00:00

abstract：:Nucleotide sequences in two wild-type and six mutant operators in the DNA of phage lambda are compared. Strikingly similar 17 base pair units are found which we identify as the repressor binding sites. Each operator contains multiple repressor binding sites separated by A-T rich spacers. Elements of 2 fold rotational ...

journal_title：Cell

authors： Maniatis T,Ptashne M,Backman K,Kield D,Flashman S,Jeffrey A,Maurer R

更新日期：1975-06-01 00:00:00

abstract：:The 5' terminal structure of the mRNA synthesized in vitro by the virion-associated RNA polymerase of vesicular stomatitis virus in the presence of S-adenosyl-L-methione consists of 7-methyl guanosine linked to 2'-O-methyl adenosine through a 5'-5' pyrophosphate bond as m7G(5')ppp(5')A-m-p ... The alpha and beta phosp...

journal_title：Cell

authors： Abraham G,Rhodes DP,Banerjee AK

更新日期：1975-05-01 00:00:00

abstract：:The rate of incorporation of leucine into protein, the rate of polypeptide elongation and termination, and the relative quantity and size of polysomes were analyzed in mouse L cells grown in suspension culture at various temperatures between 0 degrees C and 36 degrees C. Between 10 degrees C and 36 degrees C protein s...

journal_title：Cell

authors： Craig N

更新日期：1975-04-01 00:00:00

abstract：:In extracts from rat and calf uterus, the steroid hormone 17 beta-estradiol stimulates the binding of its specific receptor protein to DNA. This interaction appears to be of low affinity (half of the estradiol-activated, 5S receptor bound at 300-400 mug/ml DNA) and nonspecific with respect to DNA base sequence. No bin...

journal_title：Cell

authors： Yamamoto K,Alberts B

更新日期：1975-04-01 00:00:00